ClonExpress II One Step Cloning Kit 50 rxn. Home Shop ClonExpress II One Step Cloning Kit 50 rxn. ClonExpress technology is a simple fast and efficient DNA seamless cloning technology which can clone the insert into any position of any vector. Linearize the vector and place the insert 5’in the forward/reverse PCR primer Introduce
HiScript II One Step qRT PCR SYBR Green Kit Vazyme Biotech Co. Ltd HiScript II One Step qRT PCR SYBR Green Kit uses the SYBR Green I chimeric fluorescence method and is designed for quantitative PCR detection using RNA as a template such as RNA viruses . Using gene specific primers GSP reverse transcription and PCR reactions are completed in one tube no additional tube opening
Jul 01 2021 ClonExpress R Ultra One Step Cloning Kit V azyme Biotech. Co. Ltd the target genes were ligated to the pBI121 vector. The ligation products were transformed into E. coli strain DH5
Buy One Get One Free from 15. Feb. 2021 to 15. Apr. 2021. The promotional price will be included in the order confirmation. ClonExpress II One Step Cloning Kit 73.00 ClonExpress Ultra One Step Cloning Kit 97.00
For wheat the full length CDS of TaSG D1 and Tasg D1of HS2 and ND4332 were amplified using specific primer pairs and inserted into the pWMB110 vector via homologous recombination technology with a ClonExpress II One Step Cloning Kit Vazyme .
The three fragments plasmid skeleton trcO RBS and BBa B0015 terminator sharing the overlap one by one were assembled together by ClonExpress Ultra One Step Cloning Kit Vazyme Biotech . The new plasmid was named as p15A trcO3415.
qScript XLT One Step RT qPCR ToughMix qScript XLT One Step RT qPCR ToughMix is an easy kit that contains all the reagents needed for the master mix in one tube. The only components I added were my primers probe and nuclease free water. The protocol is short and user friendly.
C112 02 ClonExpress II One Step Cloning Kit 50 tests size 50 reactions 436.03 USD
Jul 01 2021 The DG1G01828.1 CDS region was inserted into in the pYES2 vector using the ClonExpress Ultra One Step Cloning kit Vazyme Nanjing China for expression in yeast. In groups II AtSPL1/12 AtSPL14/16 PvSPL14/16 and three DgSPL genes were
ClonExpress UItra One Step Cloning kit Vazyme #C115 is a novel cloning kit applicable for homologous recombination with 15 fragments. The Kit is independent of DNA ligase significantly reducing the self ligated colonies. The enhanced Exnase and highly optimized buffer included in 2x ClonExpress
Apr 02 2021 HiScript II Q RT SuperMix for qPCR R222 01 2 Taq Master Mix P212 01 and ClonExpress Ultra One Step Cloning Kit C115 01 were obtained from Vazyme P.R. China. . Inhibitors including amlodipine HY B0317 Go6983 HY 13689 AZ628 HY 11004 and U0126 HY 12031 were purchased from MedChemExpress USA .
ClonExpress II One Step Cloning Kit ClonExpress Ultra One Step Cloning Kit . 5min Universal Ligation Mix 1 1 1 BioLinks Lab Supplies. Nijenheim 1325. 3704VC Zeist. The Netherlands. Tel . Email info bio links. Reviews. Rating 3. stars
Molecular cloning was performed using either ClonExpress Ultra One Step Cloning Kit Vazyme #C115 02 or CloneEZ PCR Cloning Kit Genscript #L00339 . Amino acid substitutions and the deletion mutants were generated by PCR based methods.
NB ClonExpress Ultra One Step Cloning Kit. Reagents and instruments for immunology cell biology and molecular biology. ClonExpress II One Step Cloning Kit. 50rxn . NB. ClonExpress MultiS One Step Cloning Kit. 25rxn . NB. Phanta Max Super Fidelity DNA Polymerase. 100U
ClonExpress Ultra One Step Cloning Kit is a new generation of recombinant cloning kit which can quickly and efficiently clone 1 5 fragments to any position of any vector. The highly optimized 2 ClonExpress Mix 50℃ reaction 5 15 Min completes the reorganization and
ClonExpress II One Step Cloning Vazyme #C112 is a simple fast and high efficient cloning technology which is based on a homologous recombination technology. It enables directional insertion of any amplified DNA product into any linearized vector at any site. Firstly the vector is linearized at the cloning
C112 02 ClonExpress II One Step Cloning Kit. Reagents and instruments for immunology cell biology and molecular biology. Log in . Forgot your password Open account. ClonExpress Ultra One Step Cloning Kit. 50rxn Wilhelm Mauser Str. 41 43 50827 KölnGermany Tel 49 221
Allele In One Mouse Tail Direct Pcr System Allele In One Mouse Tail Direct Pcr Kit Igm Is One Of The First Types Of Antibodies All In One Pcr Mastermix Clonexpress Ii One Step Cloning Ki Clonexpress Ii One Step Cloning Kit Clonexpress Ii One Step Cloning Kit Cells Direct One Step Qrt Pcr Kit Accurate Chemicals One Step Cell Direct One
C112 01 ClonExpress II One Step Cloning Kit. Antibodies Recombinant proteins ELISA kits RNAi cDNA clones Antibody Array Luminex kits. Reagents and instruments for immunology
Dec 01 2018 ClonExpress II One Step Cloning Kit was purchased from Vazyme Vazyme Biotech Co. Ltd Nanjing China . All other reagents and solvents are of analytical grade and purchased from Sinopharm Chemical Reagent Co. Ltd Shanghai China . 2.2. Plasmid construction.
Nov 23 2020 Lab Reagents . Human IgG antibody Laboratories manufactures the vazyme biotech reagents distributed by Genprice. The Vazyme Biotech reagent is RUO Research Use Only to test human serum or cell culture lab samples.
C112 02 ClonExpress II One Step Cloning Kit. Reagents and instruments for immunology cell biology and molecular biology.
The insert and the linearized vector with overlapped sequences of 15 bp20 bp on both 5’ and 3’ end respectively are mixed in an appropriate ratio and incubated with recombinase Exnase at 50℃ for 515 min.The Vazyme ClonExpress UItra One Step Cloning kit is a novel cloning kit applicable for homologous recombination with 1
Aug 18 2020 pRS42K was the basis of the expression vector. pRS42K was double digested with SalI and EcoRI Thermo Fisher Scientific USA and recombined with the promotor TEF1p terminator CYC1t Gfegt1 and Gfegt2 by using the ClonExpress Ultra One Step Cloning Kit Vazyme China .
C112 02 ClonExpress II One Step Cloning Kit. Reagents and instruments for immunology cell biology and molecular biology.
digestion and one step cloning ClonExpress II One Step Cloning Kit C112 02 Vazyme Biotech Nanjing China or recombinant methods. The 39 UTR sequences of RBM5 containingwild type WT ormutant mut formsofthe miR 432 5p target site were inserted into the restriction endonucleases XhoI Xho1 and restriction endonucleases
Dec 07 2020 The putative transposition unit of tet X3 in Clade U6 10FS3 1 namely ΔtpnF tet X3 hp hp ISCR2 was cloned in a suicide plasmid pSV03 using the ClonExpress Ultra One Step Cloning Kit Vazyme China Additional file 1 Table S1 and then transformed into competent E. coli WM3064.
C115 02 ClonExpress Ultra One Step Cloning Kit. Reagents and instruments for immunology cell biology and molecular biology. ClonExpress II One Step Cloning Kit. 50rxn . C113 01. ClonExpress MultiS One Step Cloning Kit
Feb 01 2020 Full length enhanced green fluorescent protein EGFP and TgLITAF ORF were amplified by PCR using the primers listed in Table 1 and subcloned into pcDNA3.1 vector to construct the pcDNA EGFP pcDNA TgLITAF pcDNA EGFP TgLITAF recombinant plasmids by the ClonExpress Ultra One Step Cloning Kit. All constructed plasmids derived from PCR
ClonExpress II One Step Cloning Kit. 50rxn . C113 01. ClonExpress MultiS One Step Cloning Kit. 10rxn . C113 02. ClonExpress MultiS One Step Cloning Kit. 25rxn
Jun 29 2018 Gene recombination was performed using the ClonExpress II One Step Cloning Kit Vazyme . To generate pLEX NLRP3 C275A primers were
Aug 21 2019 A ClonExpress Ultra One Step Cloning Kit Vazyme China was utilized to introduce mutations in selected AAV1 or AAV5 capsid residues at the
Jun 27 2021 We used a two fragment assembling method for construction of plasmids pQFA14 pQFA22 according to the guideline of ClonExpress Ultra One Step Cloning Kit Vazyme Nanjing China and the primers were listed in Additional file 1 Table S1. All expression vectors harboring two genes were constructed in bicistronic pattern.
2020 Product Catalogue Reagents for Molecular Biology Research Contents Contents Product List 01 Applications 06 PCR 07 Cloning Mutagenesis 11 Nucleic Acid Electrophoresis 14 Reverse Transcription 15 qPCR 17 Nucleic Acid Isolation 20 Cell Biology Protein Research 24 Genome Editing 28 Selected Product Citations 29
ClonExpress Ultra One Step Cloning Kit ClonExpress UltraOneStepCloningKit 5 15min Mix 50bp 10kb >95
C115 02 ClonExpress Ultra One Step Cloning Kit. Reagents and instruments for immunology cell biology and molecular biology. ClonExpress II One Step Cloning Kit. 50rxn . C113 01. ClonExpress MultiS One Step Cloning Kit
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