ClonExpress technology is a simple fast and efficient DNA seamless cloning technology which can clone the insert into any position of any vector. Linearize the vector and place the insert 5 in the forward/reverse PCR primer Introduce the end sequence of the linearized vector into the end so that the 5 and 3 ends of the PCR product have the same sequence as the two ends of the linearized
ClonExpress Ultra One Step Cloning Kit The ClonExpress cloning technology is simple fast and high efficient which enables directional insertion of any
Jan 31 2015 New England Biolabs announces the introduction of the NEBuilder HiFi DNA Assembly Cloning Kit and Master Mix for one step cloning and multiple DNA fragment assembly in
C115 01 ClonExpress Ultra One Step Cloning Kit. Reagents and instruments for immunology cell biology and molecular biology.
The TA Cloning Kit with pCR 2.1 vector provides a quick one step cloning strategy for directly inserting a Taq amplified PCR product into a plasmid vector.The TA Cloning Kit uses the pCR 2.1 cloning vector and ExpressLink T4 DNA Ligase to generate a ligation product in a fifteen minute room temperature ligation step.
Jul 21 2021 Seamless cloning kit ClonExpress Ultra One Step Cloning Kit high fidelity DNA polymerase Phanta Super Fidelity DNA Polymerase and point mutation kit Mut Express II Fast Mutagenesis Kit V2 were provided by Vazyme Nanjing China . Restriction endonuclease and T4 DNA ligase were provided by TaKaRa Beijing China .
ClonExpress MultiS One Step Cloning kit is a new version cloning kit based on ClonExpress One Step Cloning technology. Exnase MultiS and reaction buffer supplied in this kit are especially optimized for multi insertion seamless cloning MultiS for short . With the help of this kit sequential assembly of up to five insertions can be realized in
IPTG Ultra Pure 25g BioShop View Detail Add to Order. Delivered in 3 to 5 days estimated 306.66 . . ClonExpress II One Step Cloning Kit 25 rxn Vazyme View Detail Add to Order. Delivered in 1 to 2 days estimated 156.40 C113 01 . C113 01. Virtual.
Nov 23 2020 ClonExpress Ultra One Step Cloning Kit C115 01 Vazyme 25 rxn EUR 193 ClonExpress Ultra One Step Cloning Kit C115 02 Vazyme 50 rxn EUR 251 Search for Recent Posts. Establishment of reference intervals for pediatric complete capillary blood counts A
ClonExpress Ultra One Step Cloning Kit Vazyme Biotech Co. Ltd ClonExpress Ultra One Step Cloning Kit is a new generation of recombinant cloning kit which can quickly and efficiently clone 1 5 fragments to any position of any vector. The highly optimized 2 ClonExpress Mix 50℃ reaction 5 15 Min completes the reorganization and the operation is simple and quick.
Apr 01 2021 The PCR product was inserted into the pET30a vector digested with Nde I and Xho I using a ClonExpress Ultra One Step Cloning Kit Vazyme C115 to generate pET30a 6 His SlHY5.
ClonExpress Ultra One Step Cloning Kit. The ClonExpress cloning technology is simple fast and high efficient which enables directional insertion of any amplified DNA product into anylinearized vector at any site. Firstly the vector is linearized at the cloning site. A small sequence overlapped with each end of the cloning site isadded onto the insert through PCR.
C115 02 ClonExpress Ultra One Step Cloning Kit. Reagents and instruments for immunology cell biology and molecular biology.
and XhoI NEB via ClonExpress Ultra One Step Cloning Kit Vazyme . After Sanger sequencing 100 mL of overnight E. coli culture harboring the 35S GmJAG1 3Avi plasmid Fig. S1 was used for plasmid prepa ration by the Plasmid Maxi Kit QIAGEN and the
ClonExpress Ultra One Step Cloning Kit. 50rxn Wilhelm Mauser Str. 41 43 50827 KölnGermany Tel 49 221 Fax 49 221 E mail info biotrend >> Worldwide offices and Distributors >> Other information. How to order Terms and conditions. Job vacancies
ClonExpress Ultra One Step Cloning Kit . 5min Universal Ligation Mix 1 1 1 BioLinks Lab Supplies. Nijenheim 1325. 3704VC Zeist. The Netherlands. Tel . Email info bio links. Reviews. Rating 3. stars . 1 star 2 stars 3 stars 4 stars 5 stars. Submit rating. 11 votes
Subsequently the amplicon was ligated into vector pET28H using a ClonExpress Ultra One Step Cloning Kit Vazyme Biotech. Co. Ltd. Jiangsu China to obtain the plasmid which was transformed into E. coli BL21 DE3 competent cells.
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Apr 01 2021 The PCR product was inserted into the pET30a vector digested with Nde I and Xho I using a ClonExpress Ultra One Step Cloning Kit Vazyme C115 to generate pET30a 6 His SlHY5. The recombinant plasmid was transformed into Escherichia coli BL21 DE3 competent cells and the expression of the recombinant protein was then induced with 1 mM
ClonExpress MultiS One Step Cloning Kit. 63.00 €131.00 €. excl. VAT plus shipping. Size. Choose an option 10 RXNS 25 RXNS. Clear. ClonExpress MultiS One Step Cloning Kit quantity. Add to cart. Categories Cloning Mutagenesis Fast Cloning Molecular Biology SKU C113.
ClonExpress Ultra One Step Cloning Kit C115 01 Vazyme 25 rxn EUR 193 ClonExpress Ultra One Step Cloning Kit C115 02 Vazyme 50 rxn EUR 251 RealScreen CRP Ultra Sensetive ELISA Kit EL GenDepot 96T EUR 968 Human ultra Estriol uE3 ELISA KIT ELA E0919h Lifescience Market 96 Tests EUR 824 Human ultra Estriol uE3 ELISA Kit
pCE Zero Vector Linearized is supplied with ClonExpress Ultra One Step Cloning Kit Vazyme #C115 . It is highly recommended to use Vazyme s APP CEDesign for easy primer design. Single fragments and multiple fragments were cloned or assembled to pCE Zero Vector using ClonExpress Ultra One Step Cloning Kit Vazyme #C115 respectively.
ClonExpress II One Step Cloning Kit Vazyme Biotech Co. Ltd . Typically 20 μL of a mixture containing 4 μL of 5 CE II Buffer 2 μL of Exnase II 50 ng linear plasmid 100 ng PCR fragment and ddH2O was incubated at 37oC for 30 min and the resulting solution was used to transform chemically competent E. coli DH5α cells. Colony PCR and
ligated into the pET 28a vector Novagen using a ClonExpress Ultra One Step Cloning Kit Table S3 . The intron free nsrK and nsrQ genes were prepared by amplifying and ligating the two predicted exons in which the sequence of nsrK in the database was revised Table S5 . Since the nsrO gene does not have an intron it
C115 02 ClonExpress Ultra One Step Cloning Kit. Reagents and instruments for immunology cell biology and molecular biology.
May 20 2021 The gene of these enzymes were synthesized according to the codon preference of the P. pastoris expression system and then were fused into expression plasmid pPICZαa by the ClonExpress Ultra One Step Cloning Kit Vazyme Nanjing China avoiding the additional introduction of the restriction endonuclease cleavage sites.
ClonExpress Ultra One Step Cloning Kit. 25rxn . C115 02. ClonExpress Ultra One Step Cloning Kit. 50rxn C Hermanos del Moral 13 Bajo E 28019 MadridSpain Tel. 34 91 269 40 65 Fax 34 91 269 40 74 pedidos quimigen quimigen. Other information. How to order Terms and conditions. Job vacancies
Clontech Laboratories provides kits reagents and services to help you explore questions about gene discovery regulation and function
ClonExpress II One Step Cloning Kit ClonExpress IIOneStepCloningKit 50bp 10kbDNAPCR >95
Jul 06 2021 To generate GLO overexpression transgenic lines each GLO sequence was cloned into the pYLox.5 vector using the ClonExpress Ultra One Step Cloning Kit Vazyme . For the generation of CRISPR Cas9 knockout lines specific targeting sequences of GLO1 and GLO4 Additional file 8 were synthesized and cloned into the pYLCRISPR/Cas9Pubi vector .
Visit ChemicalBook To find more ClonExpress MultiS One Step Cloning Kit information like chemical properties Structure melting point boiling point density molecular formula molecular weight physical properties toxicity information customs codes. You can also browse global suppliers vendor prices Price manufacturers of ClonExpress MultiS One Step Cloning Kit .
ClonExpress Ultra One Step Cloning Kit was purchased from Vazyme Nanjing China . MonPure Gel PCR Clean Kit and Plasmid Miniprep Kit were bought from Baisai Biotechnology Qingdao China . GelRed for agarose gel electrophoresis was purchased from the MDBio Xinbei China .
Dec 07 2020 The putative transposition unit of tet X3 in Clade U6 10FS3 1 namely ΔtpnF tet X3 hp hp ISCR2 was cloned in a suicide plasmid pSV03 using the ClonExpress Ultra One Step Cloning Kit Vazyme China Additional file 1 Table S1 and then transformed into competent E. coli WM3064.
BamH1 cloning site using ClonExpress Ultra One Step Cloning Kit Vazyme cat. # C115 01 . Yeast strains and plasmids Saccharomyces cerevisiae EBY 100 was used as the host strain for the yeast surface display experiment. The plasmid pYD2 encodes β glucosidase gene and an anchor
Apr 02 2021 The PCR products together with the pcDNA3.1 vector were digested and ligated by the ClonExpress Ultra One Step Cloning Kit 34. pcDNA ERK1 and pcDNA SOX9 S64A/E S149A/E and S181A/E were subcloned from pMT ERK1 and wild type WT pcDNA SOX9 by General Biosystems.
The NUP210 CDS sequence was cloned into the pGL3 vector using the ClonExpress Ultra One Step Cloning kit Vazyme Nnajing China . miR 22 mimics from GenePharma and pGL3 NUP210 were co transfected into 293T cells using Lipofectamine 2000 Thermo Fisher according to
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